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abdulbasitbehlim/README.md
Abdul Basit Behlim wave header Abdul Basit Behlim - Data Scientist, Computational Biology and Bioinformatics

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Typing animation: Computational Biology, Bioinformatics, AI and ML for Biological Systems

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Hello, welcome to my GitHub Account πŸ‘‹

I work at the intersection of biology, bioinformatics, data science, machine learning, and scientific software. My focus is on building practical computational tools that help turn biological questions into transparent and reproducible workflows.

Here, you will find projects spanning phage biology, CRISPR, microbial genomics, structural bioinformatics, bioprocess modelling, and AI/ML for biotechnology.

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About me

I am an M.Sc. Biotechnology student specialization in Industrial Biotechnology at Gujarat Biotechnology University working at the intersection of biology, data science, bioinformatics, and research software.

My work focuses on turning biological questions into transparent, reproducible, and practical computational workflows. I am especially interested in microbial genomics, phage biology, antimicrobial research, genome engineering, structural bioinformatics, and machine-learning applications in biotechnology.

Current direction: using data and biology together to build tools that are scientifically useful, interpretable, and reproducible.

What I work on

  • 🦠 Phage & microbial bioinformatics - phage annotation, endolysin discovery, sequence analysis, protein representations, and host prediction
  • 🧬 Genome engineering - CRISPR guide design, multi-gene targeting, validation, and sequence provenance
  • 🧫 Bioprocess data science - soft sensors, fault-inclusive modelling, uncertainty, and out-of-distribution analysis
  • πŸ”¬ Structural bioinformatics - protein structure analysis, molecular docking, and computational modelling
  • πŸ› οΈ Scientific software - Python-based tools, Streamlit applications, reproducible pipelines, and research dashboards

Featured projects

Research-oriented SpCas9 workflow for identifying a single guide RNA that can target multiple homologous plant genes, with mismatch-aware consensus design, exon/segment-safe scanning, validation, and sequence provenance.

Live app Source

Python Streamlit Biopython CRISPR Plant Genomics

Guide-design and validation workbench for OpenCRISPR-1-compatible NGG targets, including both-strand scanning, guide-expression analysis, local specificity review, and reproducible sequence provenance.

Live app Source

Python Streamlit Biopython CRISPR Validation

Machine-learning soft sensor for estimating penicillin concentration from simulated fermentation batches, with normal-only and fault-inclusive models, batch-wise validation, uncertainty estimation, and OOD detection.

Read the IndPenSim preprint GitHub repository

Python Machine Learning Bioprocessing OOD Detection Soft Sensor

General-purpose SpCas9/CRISPRi design workbench with NCBI/Ensembl sequence retrieval, transparent guide ranking, local off-target screening, and TSS-aware CRISPRi support.

Live app Source

Python Streamlit CRISPRi NCBI Ensembl

Current research

🦠 Phage AI/ML pipeline

Developing an end-to-end computational workflow for phage-host analysis:

Metagenomic data
      ↓
Viral / phage sequence detection
      ↓
Genome annotation
      ↓
Endolysin identification
      ↓
Protein sequence representations / embeddings
      ↓
Bacterial host prediction

The objective is to combine biologically meaningful sequence information with interpretable AI/ML methods for phage-bacteria association analysis.

Technical toolkit

Core development
Python, Git, GitHub, Docker, VS Code and Linux

Data science & machine learning
NumPy Pandas SciPy scikit-learn Matplotlib Plotly Jupyter

Bioinformatics & scientific apps
Biopython Streamlit NCBI Ensembl CRISPR SpCas9

Web, testing & reproducibility
HTML, CSS and JavaScript Pytest GitHub Actions

Research snapshot

Area Details
πŸŽ“ Education M.Sc. Industrial Biotechnology, Gujarat Biotechnology University
πŸ… National rank GAT-B 2025 - All India Rank 62; DBT-supported graduate fellowship
πŸ”¬ Research experience Structure-based study of human Ξ²-cardiac myosin inhibitors at IIT Roorkee
πŸ† Recognition Best Student of the Year Award (2022-24), MBSI with HiMedia Laboratories

How I approach scientific software

I aim to keep research tools:

  • Biologically grounded - scientific assumptions and limitations are stated clearly
  • Reproducible - versions, provenance, benchmark inputs, and tests are retained
  • Interpretable - model or heuristic outputs are presented with appropriate context
  • Usable - researchers can run workflows through practical interfaces and export results
  • Open to validation - computational predictions are treated as evidence to investigate, not substitutes for experimental confirmation

Additional projects

Connect

I am interested in research collaborations where biotechnology, computational biology, bioinformatics, machine learning, and practical scientific software come together.

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Thank you for visiting my profile
Biotechnology Γ— AI/ML for Biological Systems Γ— Computational Biology Γ— Bioinformatics

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  1. IndPenSim-penicillin-soft-sensor IndPenSim-penicillin-soft-sensor Public

    Machine-learning soft sensor for estimating penicillin concentration using the IndPenSim simulation dataset, with baseline and fault-inclusive models, batch-wise validation, uncertainty estimation,…

    Jupyter Notebook 1

  2. CRISPR-gRNA-Designer CRISPR-gRNA-Designer Public

    A Python/Streamlit tool that turns a gene name and organism into ranked SpCas9 guide RNAs for CRISPR knockout or knockdown.

    Python

  3. Plant-MultiGene-gRNA-Designer Plant-MultiGene-gRNA-Designer Public

    A Python/Streamlit tool for designing and validating single SpCas9 gRNAs that can target multiple homologous plant genes, with exact and mismatch-aware multi-gene guide discovery.

    Python

  4. OpenCRISPR1-gRNA-Designer OpenCRISPR1-gRNA-Designer Public

    A Python/Streamlit tool for designing and validating NGG-compatible gRNAs for OpenCRISPR-1, with guide-format analysis, local off-target screening, and reproducible sequence provenance.

    Python